Galina Zhuravleva

Geneticheskiy Kontrol' Terminatsii Translyatsii U Eukariot: Identifikatsiya Belkov Erf3/sup35 I Pab1, Kak Uchastvuyushchikh V Protsesse Terminatsii Galina Zhuravleva Russian edition

Biosintez belka, ili translyatsiya, yavlyaetsya vazhneyshim matrichnym protsessom, otvetstvennym za realizatsiyu geneticheskoy informatsii. Za proshedshie gody dostignut znachitel'nyy progress v ponimanii togo, kak osushchestvlyaetsya protsess sinteza belka v kletke, kakim obrazom on integriruetsya s drugimi matrichnymi protsessami, takimi kak replikatsiya i transkriptsiya, kak osushchestvlyaetsya geneticheskiy kontrol' translyatsii. Eta kniga posvyashchena kharakteristike protsessa terminatsii u eukarioticheskikh organizmov. Primenenie model'nogo ob"ekta, drozhzhey Saccharomyces cerevisiae, pozvolilo detal'no okharakterizovat' protsess terminatsii translyatsii u eukariot. Identifitsirovany belki, uchastvuyushchie v etom protsesse, v tom chisle faktor terminatsii translyatsii eRF3, kodiruemyy u drozhzhey genom SUP35. Pokazano, chto funktsii belka eRF3 konservativny u razlichnykh eukarioticheskikh organizmov, takikh kak S. cerevisiae, X. laevis, M. musculus, H. sapiens. Obnaruzheno, chto v khode terminatsii translyatsii belok eRF3 vzaimodeystvuet s belkom Rab1 (poly-A-binding protein, PABP u vysshikh eukariot), identifitsirovany domeny oboikh belkov, uchastvuyushchie v etom vzaimodeystvii. Pokazano, chto vzaimodeystvie eRF3 - RAVR konservativno v khode evolyutsii.