Anita Tilwari

Genetic Diversity of Medicinal Plant Using Rapd Markers: Evaluation of Rapd Markers for Assessment of Genetic Variation in Ocimum Sanctum: an Important Medicinal from India Anita Tilwari

The RAPD markers are an efficient tool for assessing the genetic diversity and are less expensive then molecular techniques like AFLP, RFLPs etc. therefore more convenient for use in research. This book basically covers the basic methodology used for assessing the genetic diversity like DNA isolation,quantitative and qualitative Estimation of DNA, RAPD-PCR, Agarose gel electrophoresis, Gel documentation,and use of Bioinformatics tool for data analysis. Rapid molecular testing techniques for the verification of elite genotypes/cultivars identity assume importance in the face of demands for exacting quality in international trade, need for ensuring homogeneity in planting stocks and protection of intellectual property rights on indigenous biodiversity of O. sanctum present in central India. The present study has shown the usefulness of RAPD markers for diversity analysis of O sanctum genotypes as well as cultivars obtained from different localities, particularly identification of the specific bands may be considered important in the identification and for conservation of species and may lead to planning of a better management of conservation program in the country.